Reverse Transcription:Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS
Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. WT BALB/c mice, WT C57BL/6J mice, immunodeficient BALB/c-nude mice, CD11c-dtr mice (Jackson Laboratory, B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J) and NCG mice (GemPharmatech, NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt) were housed under specific pathogen-free conditions at a temperature of 18 C–24 Cwith water and food and kept on 12-h light/dark cycles.
One Step RT-PCR:Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS
Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. WT BALB/c mice, WT C57BL/6J mice, immunodeficient BALB/c-nude mice, CD11c-dtr mice (Jackson Laboratory, B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J) and NCG mice (GemPharmatech, NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt) were housed under specific pathogen-free conditions at a temperature of 18 C–24 Cwith water and food and kept on 12-h light/dark cycles.
Cell Isolation:Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS
Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. WT BALB/c mice, WT C57BL/6J mice, immunodeficient BALB/c-nude mice, CD11c-dtr mice (Jackson Laboratory, B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J) and NCG mice (GemPharmatech, NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt) were housed under specific pathogen-free conditions at a temperature of 18 C–24 Cwith water and food and kept on 12-h light/dark cycles.
Enzyme-linked Immunosorbent Assay:Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS
Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. WT BALB/c mice, WT C57BL/6J mice, immunodeficient BALB/c-nude mice, CD11c-dtr mice (Jackson Laboratory, B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J) and NCG mice (GemPharmatech, NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt) were housed under specific pathogen-free conditions at a temperature of 18 C–24 Cwith water and food and kept on 12-h light/dark cycles.
RNA Sequencing:Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS
Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. WT BALB/c mice, WT C57BL/6J mice, immunodeficient BALB/c-nude mice, CD11c-dtr mice (Jackson Laboratory, B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J) and NCG mice (GemPharmatech, NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt) were housed under specific pathogen-free conditions at a temperature of 18 C–24 Cwith water and food and kept on 12-h light/dark cycles.
Multiple Displacement Amplification:Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS
Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. WT BALB/c mice, WT C57BL/6J mice, immunodeficient BALB/c-nude mice, CD11c-dtr mice (Jackson Laboratory, B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J) and NCG mice (GemPharmatech, NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt) were housed under specific pathogen-free conditions at a temperature of 18 C–24 Cwith water and food and kept on 12-h light/dark cycles.
Recombinant:Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS
Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. WT BALB/c mice, WT C57BL/6J mice, immunodeficient BALB/c-nude mice, CD11c-dtr mice (Jackson Laboratory, B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J) and NCG mice (GemPharmatech, NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt) were housed under specific pathogen-free conditions at a temperature of 18 C–24 Cwith water and food and kept on 12-h light/dark cycles.
Software:Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 OPEN ACCESS OPEN ACCESS
Article Title: Thymosin α1 reverses oncolytic adenovirus-induced M2 polarization of macrophages to improve antitumor immunity and therapeutic efficacy
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC Cat#CRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiScript Reverse Transcriptase reagent kit Vanzyme Cat#R101-02 HiScript II One Step RT-PCR Kit Vanzyme Cat#P611-01 Adeno-XTM rapid titer kit Clontech Cat#632250 CD4+ T cell isolation kit, mouse Selleck Cat#B90000 CD8+ T cell isolation kit, mouse Selleck Cat#B90011 Mouse IL-1b ELISA kit Elabscience Cat#E-EL-M0046c Human IL-1b ELISA kit Elabscience Cat#E-EL-H0149 Mouse IL-10 ELISA kit Elabscience Cat#E-EL-H0149c Human IL-10 ELISA kit Elabscience Cat#E-EL-H6154 Deposited data 4T1 cells RNA sequencing GEO database GEO: GSE271046 Macrophages RNA sequencing GEO database GEO: GSE271202 Experimental models: Cell lines 4T1 cells ATCC Cat#CRL-2537 CT26 cells ATCC CatCRL-2638 H22 cells This paper N/A B16-F10 ATCC Cat#CRL-6475 MDA-MB-231 cells ATCC Cat#CRM-HTB-26 HCT116 cells ATCC Cat#CCL-247EMT HepG2 cells ATCC Cat#HB-8065 MET-5A cells ATCC Cat#CRL-9444 THP-1 cells ATCC Cat#TIB-202 HEK 293T cells ATCC Cat#CRL-1573 RAW264.7 cells ATCC Cat#TIB-71 Experimental models: Organisms/strains Mouse: BALB/c GemPharmatech SN#000651 Mouse: Outbred athymic nude GemPharmatech SN#007850 Mouse: C57BL/6J GemPharmatech SN#000664 Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J Jackson Laboratory SN#004509 Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt GemPharmatech SN#T001475 DH5a Vanzyme Cat# C502-02 GFP+ Escherichia coli This paper N/A Oligonucleotides See Table S1 for primers N/A N/A Recombinant DNA pAd-DEST This paper N/A pAd-Ta1 This paper N/A Software and algorithms GraphPad Prism 9 N/A N/A FlowJo 10.7.1 Tree Star N/A Adobe Illustrator Adobe N/A BioRender BioRender N/A e2 Cell Reports Medicine 5, 101751, October 15, 2024 Article ll OPEN ACCESS Article ll OPEN ACCESS .. WT BALB/c mice, WT C57BL/6J mice, immunodeficient BALB/c-nude mice, CD11c-dtr mice (Jackson Laboratory, B6.FVB-1700016L21RikTg(Itgax HBEGF/EGFP)57Lan/J) and NCG mice (GemPharmatech, NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt) were housed under specific pathogen-free conditions at a temperature of 18 C–24 Cwith water and food and kept on 12-h light/dark cycles.
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